Fig. 1

Tumor-forming ability of PCa cells in bone and the expression levels of KLF7/CCL2 in the bone under the conditions of obesity. BALB/c nude mice were fed a 60% high-fat diet. A Schema for the bone marrow PCa-bearing mouse model in obese state. B The weight and (C) Lee’s index were determined for these mice. In the fourth week, fluorescent PC-3-Luc cells were injected into the femur of the left leg of each mouse. Subsequently, (D) the Small Animal Imaging Technology was applied. After the H&E staining of the femur of the left leg of the mice, (E, Up) the BMA cells with a size of over 50 µm were observed under a microscope at a magnification of 200 × and using the CellSens standard software. (E, Down) The number of BMAs, area of a single BMA, and proportion of the BMA area in the bone marrow cavity were determined. PA (F) and CCL2 (G) levels in mouse serum were determined using ELISA. H The mRNA expression levels of HSL/ATGL in bone marrow were determined using qRT-PCR. The protein expression levels of KLF7 (I) and CCL2 (J) in BMA were determined using immunohistochemistry. K The immunohistochemistry score of KLF7 and CCL2. L The mRNA expression levels of HSL/ATGL in bone marrow were determined using qRT-PCR. M The protein expression level of CCR2 in mouse`s tumor tissue were determined using Western Blot. In the non-parametric rank-sum test, the differences with *P < 0.05, **P < 0.01, and ***P < 0.001 were statistically significant