Fig. 4

FERMT1 exerts a positive regulatory effect on PKP3 in NSCLC cells. FERMT1 mRNA level was positively corrected with PKP3 by GEPIA. (B) Predicting the mRNA level of PKP3 in NSCLC involved the utilization of GTEx and TCGA datasets. (C and D) Further analysis of PKP3 mRNA levels in LUAD and LUSC was conducted based on GTEx and TCGA datasets (C) and GEPIA (D). (E) LUAD and LUSC patients’ overall survival was assessed according to the mRNA level of PKP3. (F) RT-qPCR detected the mRNA levels of PKP3 in thirty-paired NSCLC samples. (G) FERMT1 mRNA level was positively related with PKP3 in thirty-paired NSCLC samples. (H) To detect PKP3 expression in FERMT1 overexpression or knockdown cells, Western blotting was employed. (I and J) Quantifying the protein levels of FERMT1 and PKP3 was performed in (I). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001