Fig. 1

FERMT1 is upregulated in NSCLC. (A) Analysis of the mRNA level of FERMT1 in NSCLC from GTEx dataset (https://www.gtexportal.org/home/datasets) and TCGA dataset (https://portal.gdc.com). (B and C) The mRNA levels of FERMT1 in LUAD and LUSC were further scrutinized according to GEPIA (http://gepia.cancer-pku.cn/) (C) and TCGA and GTEx datasets (B). (D) The overall survival of LUAD and LUSC patients according to the transcriptional level of FERMT1. (E) RT-qPCR detected the mRNA levels of FERMT1 in thirty-paired NSCLC samples. (F) Western blotting results of FERMT1 expression in four paired NSCLC samples. (G) RT-qPCR was employed to measure the mRNA levels of FERMT1 in four cancer cell lines mixed squamous cell carcinoma and adenocarcinoma (NCI-H226, SK-MES-1, A549, H358, and H157) and the human normal lung cell line BEAS-2B. (H) Western blotting results of FERMT1 expression in BEAS-2B, NCI-H226, SK-MES-1, A549, H358, and H157. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001