Fig. 3

The results of the analysis aimed at evaluation of sl-pHCs’ morphology and ultrastructure. Basic cellular features, including nuclei, cytoskeleton, and mitochondrial network, were captured under a confocal microscope in sl-pHCs. In contrast, cell attachment to the surface and intercellular connections were monitored under SEM. The analysis was performed for RAB-1 (a), HAN-1 (b), and CHR-1 (c). Moreover, the mitochondrial network was reconstructed in 3D, and its cellular distribution and morphology were studied (d). The comparative analysis determined the characteristic pattern of mitochondrial morphology in the obtained sl-pHCs primary cell lines. Scale bars are indicated in the representative microphotographs. Results are presented as a column with bars representing means ± SD. *** p-value < 0.001, and ****p-value < 0.0001