Fig. 4
From: Co-expression of IL-21-Enhanced NKG2D CAR-NK cell therapy for lung cancer
IL-21 enhanced proliferation potency and reduced autologous apoptosis and exhaustion of CAR-NK cells. (A, B) NK-92 cells, NKG2D CAR-NK-92 cells, and NKG2D-IL-21 CAR-NK-92 cells labeled with CFSE, were co-incubated with A549 cells at a 1:10 ratio for 6 d. The cell counts and CFSE dilution were measured. (C, D) NK-92, NKG2D CAR-NK-92, and NKG2D-IL-21 CAR-NK-92 cells which were co-incubated with A549 at a 1:10 ratio for 6 d, the apoptosis ratio and TIM-3 expression of CAR-NK-92 cells was detected by flow cytometry. Data were representative of three independent experiments. * P < 0.05; ** P < 0.01