Fig. 2
From: Co-expression of IL-21-Enhanced NKG2D CAR-NK cell therapy for lung cancer
Generation and NKG2DLs expression of CAR-NK-92 cells. (A) NKG2DLs expressions in three lung cancer cell lines were quantified by flow cytometry. (B) Schematic representation of NKG2D. (C) Flow cytometry analysis of the transfection efficiencies. (D) Western blot analysis of IL-21 protein expression level in NK-92, NKG2D CAR-NK-92, and NKG2D-IL-21 CAR-NK-92 cells. € ELISA analysis of IL-21 protein expression level in NK-92, NKG2D CAR-NK-92, and NKG2D-IL-21 CAR-NK-92 cells. Data were representative for three independent experiments. *** P < 0.001; ns, not significant