Fig. 6

lnc-PSMA8-1 modulates mTOR expression via competitively binding to miR-144-3p. A: Expression of mTOR in RD and RH30 cells transfected with lnc-PSMA8-1 siRNA in combination with miR-144-3p inhibitor as detected by qRT-PCR. B: Protein levels of mTOR and p-mTOR in RD and RH30 cells transfected with lnc-PSMA8-1 siRNA in combination with miR-144-3p inhibitor as detected by Western blot analysis. C: RD and RH30 cells cotransfected with lnc-PSMA8-1 siRNA or negative control and luciferase reporter plasmid (mTOR 3’UTR-WT/MUT/NC) were subjected to the luciferase reporter assay. All the blots were cropped prior to hybridization with primary antibodies. The original blots are presented in Fig. S5