Fig. 5

lnc-PSMA8-1 interacts with miR-144-3p and regulation of mTOR by miR-144-3p. A: RD and RH30 cells cotransfected with miR-144-3p mimics or negative control and luciferase reporter plasmid (lnc-PSMA8-1-WT/MUT/NC) were subjected to the luciferase reporter assay. B: Enrichment of lnc-PSMA8-1 bound to Ago2 or IgG in RD and RH30 cell lines as measured by qRT-PCR after RIP; IgG was used as a negative control. C: Expression levels of miR-144-3p in RD and RH30 cells transfected with lnc-PSMA8-1 siRNA or negative control as determined by qRT-PCR. D: Levels of lnc-PSMA8-1 in RD and RH30 cells transfected with miR-144-3p mimics or negative control as determined by qRT-PCR. E: Enrichment of mTOR bound to Ago2 or IgG in RD and RH30 cell lines as measured by qRT-PCR after RIP; IgG was used as a negative control. F: RD and RH30 cells cotransfected with miR-144-3p mimics or negative control and luciferase reporter plasmid (mTOR 3’UTR-WT/MUT/NC) were subjected to the luciferase reporter assay. G: Expression levels of mTOR in RD and RH30 cells transfected with miR-144-3p mimics or negative control as determined by qRT-PCR. H: Protein levels of mTOR and p-mTOR in RD and RH30 cells transfected with miR-144-3p mimics or negative control as determined by Western blot analysis. All the blots were cropped prior to hybridization with primary antibodies. The original blots are presented in Fig. S3 and Fig. S4