Fig. 6

EMP3 affects the malignant phenotype of glioblastoma by promoting the EMT process (A)-(D). Correlation analysis of VIM, FOS, SNAI2, TWIST1 and EMP3 using TCGA data (E)-(H). These related EMT markers were tested by qRT-PCR after the knockdown of EMP3 for 96 h cultured with siRNA. On the other hand, proteins of EMT markers VIM, SNAI2, FOS, TWIST1 were investigated by western blot after EMP3 siRNA transient transfection (I)-(M), Actin as the internal parameter. Cells were divided into three groups, NC (negtive control) group, siRNA EMP3-1 and siRNA EMP3-2 group. U87 cells were lysis after siRNA incubation for 72 h. The efficiency of trasfection of siRNA EMP3 was detected by WB (N). All of the full-length blots/gels of western blot are presented in Supplementary Figure 1C-H. *p < 0.05, **P < 0.01, ***P < 0.001,****p < 0.0001