Fig. 5

Knockdown of EMP3 inhibits the malignant phenotypes of glioblastomas (A) and (B). The expression of EMP3 was significantly reduced after transfection of U87 and U251 cells for 72 h cultured with siRNA, and GAPDH was used as an internal control (C) and (D). The proliferation rate of U87 and U251 cells in the si-EMP3 group was significantly lower than that of the NC group at 24 h, 48 h, 72 h (E). The number of migrating U87 and U251 cells in the si-EMP3 group was lower than that in the NC group at 72-96 h (F). Wound healing results indicated the migration capacity of the si-NRP1 group was significantly lower than that of the NC group at 72-96 h.The knockdown efficiency of EMP3 in U251 glioma cells are detected by western blot (G). Cells were divided into four groups, NC (negtive control) group, siRNA EMP3-1, siRNA EMP3-2 and siRNA EMP3-3 group. U251 cells were lysis after siRNA incubation for 72 h. All of the full-length blots/gels of WB are presented in Supplementary Figure 1A and B. *p < 0.05, **P < 0.01, ***P < 0.001, ****p < 0.0001