Fig. 3
From: UTP11 promotes the growth of hepatocellular carcinoma by enhancing the mRNA stability of Oct4
UTP11 promotes the growth of liver cancer cells in vitro. (A) The mRNA expression levels of UTP11 in normal human liver cell line (HL-7702) and liver cancer cell lines (Bel-7404, Huh7, HepG2, HCC-LM3) were detected by qRT-PCR assay. (B) The mRNA levels of UTP11 in control or UTP11-knockdown HepG2 and HCC-LM3 cells were examined by qRT-PCR assay. (C) The protein expression of UTP11 in control or UTP11-knockdown HepG2 and HCC-LM3 cells were tested using western blotting assay. (D) The viability of control or UTP11-knockdown HepG2 and HCC-LM3 cells was checked using CCK8 assay. (E) The proliferation was detected using EdU staining in control or UTP11-knockdown cells. (F) HepG2 and HCC-LM3 cells treated with shUTP11 for 7 days (UTP11 knockdown group), followed with 7-AAD staining and detected by flow cytometry. * P < 0.05; ** P < 0.01; *** P < 0.001