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Fig. 5 | BMC Cancer

Fig. 5

From: Exosomes derived from cancer-associated fibroblasts promote tumorigenesis, metastasis and chemoresistance of colorectal cancer by upregulating circ_0067557 to target Lin28

Fig. 5

Overexpression of Lin28A and Lin28B attenuated the effects of circ_0067557 silencing on the malignant behaviors of CRC cells. (A) The interaction of circ_0067557 and Lin28A or Lin28B was monitored by the RIP assay. Original gels were provided in Supplemental files. (B) RNA-pulldown was used to evaluate the combination of circ_0067557 and Lin28A or Lin28B. The interaction between circ_0067557 and Lin28A (C) or Lin28B (D) was detected by EMSA assay. (E) Drug resistance (5-FU and L-OHP) was evaluated in circ_0067557-silenced HCT116 cells transfected with Lin28A- or Lin28B-overexpressing plasmids using the CCK-8 assay. (F) HCT116 proliferation was evaluated by EdU staining. (G) Sphere-forming assay. (H) Apoptosis was monitored by flow cytometry. CD133, CD44, and OCT4 expression levels were evaluated by RT-qPCR (I) and Western blotting (J). (K) EMT and cell cycle-related proteins were detected by Western blotting

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BMC Cancer

ISSN: 1471-2407

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