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Fig. 2 | BMC Cancer

Fig. 2

From: Exosomes derived from cancer-associated fibroblasts promote tumorigenesis, metastasis and chemoresistance of colorectal cancer by upregulating circ_0067557 to target Lin28

Fig. 2

Circ_0067557 knockdown decreased Lin28A and Lin28B expression in CAF-derived exosomes. (A) RT-qPCR analysis of circ_0067557 in HCT116, SW480, and LoVo cells after co-culture with NFs and CAFs. (B) Circ_0067557 expression in HCT116, SW480, and LoVo cells was examined by RT-qPCR after exposure to exosomes from NFs and CAFs. (C) RT-qPCR analysis of circ_0067557 expression in HCT116 cells following circ_0067557 silencing. (D-E) Fluorescence of HCT116 cells treated with PKH67-labeled exosomes derived from NFs and CAFs. (F) CRC cells were transfected with si-circ_0067557 and then treated with exosomes derived from NFs and CAFs; circ_0067557 expression was measured by RT-qPCR. (G) Circ_0067557 expression in HCT116, SW480 and LoVo cells treated with si-circ_0067557 and CAF-derived exosomes was measured by RT-qPCR. Lin28A and Lin28B expression was evaluated by RT-qPCR (H) and Western blotting (I) in CRC cells treated with si-circ_0067557 and CAF-derived exosomes. (J) H&E staining of the sections of CRC tumors and adjacent normal tissues dissected from patients (n = 12). The glands were irregular with necrosis, interstitial fibrosis, infiltrating cells, and pathological mitosis in sections of CRC tumors. 20× and 40× magnification. (K) IHC showed higher expression of Lin28A and Lin28B in the sections of CRC tumors dissected from patients compared to adjacent normal tissues (n = 12). 20× and 40× magnification

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BMC Cancer

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