Exosomes derived from cancer-associated fibroblasts promote tumorigenesis, metastasis and chemoresistance of colorectal cancer by upregulating circ_0067557 to target Lin28

Yang, Cheng; Zhang, Yan; Yan, Mingze; Wang, Jiahao; Wang, Jiaming; Wang, Muhong; Xuan, Yuhong; Cheng, Haiyue; Ma, Jiaao; Chai, Cuicui; Li, Mingzhe; Yu, Zhiwei

doi:10.1186/s12885-023-11791-5

Fig. 1

From: Exosomes derived from cancer-associated fibroblasts promote tumorigenesis, metastasis and chemoresistance of colorectal cancer by upregulating circ_0067557 to target Lin28

Identification of DECs in exosomes derived from NFs and CAFs. (A) IF staining of FAP, vimentin, and α-SMA in CAFs and NFs isolated from CRC and adjacent normal tissue, respectively; 20×magnification. (B) NanoSight particle size detection of exosomes isolated from CAF and NF culture medium. (C) The morphology of exosomes secreted by CAFs and NFs was observed by TEM. (D) Western blotting for CD63, ALIX, and CNX to identify exosomes. (E) Heat map of DECs in exosomes derived from NFs and CAFs confirmed by circular RNA microarray. The upregulated and downregulated DECs were selected by the criteria of fold of change (FC) > 1.5, P < 0.05. (F) Clustered heatmap of the top 15 upregulated and downregulated circRNAs in exosomes derived from NFs and CAFs. (G) GO analysis for DECs in exosomes secreted by CAFs and NFs. (H) KEGG analysis of DECs in exosomes from CAFs and NFs. KEGG analysis was based on www.kegg.jp/kegg/kegg1.html with the permission from the Kanehisa laboratory. (I) The expression of the top five upregulated and downregulated circRNAs in exosomes derived from NFs and CAFs was confirmed by RT-qPCR. (J) Expression of circ_0067557 in cDNA or gDNA was identified using divergent primers. Original gels were provided in Supplemental files

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BMC Cancer

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