Fig. 2

MYO5A promotes migration and invasion of HNSC. A A heatmap showing the Copy Number Variation (CNV) profile of MYO5A gene in different clustered cells in HNSC using single-cell RNA-seq data from GSE103322 using CancerSEA. Rows represent hierarchically clustered cells, columns represent PCGs which ordered by genomic location (1-22 and X), and separation represents the gap of chromosomes. B Correlations between the MYO5A and functional states in HNSC using single-cell RNA-seq data from GSE103322. C The expression pattern of MYO5A by t-SNE (t-distributed stochastic neighbor embedding) and PCA (principal component analysis) analysis in HNSC (GSE103322). D Functional states that are significantly related to MYO5A in cluster MEEI 18 in HNSC (GSE103322). E The correlation of differentiation, metastasis, angiogenesis and inflammation with MYO5A. F Efficiency of siRNA knockdown of MYO5A in FaDu cell line was performed by RT-PCR (at 36 hours post-transfection) and western blot (at 48 hours post-transfection). The blots were cropped from different parts of the same gel prior to hybridisation with different antibodies. Tubulin was used as the reference. G-H Cell migration and invasion were respectively assessed by wound healing and chamber cell migration assays. At 36 hours post-transfection, FaDu cells were seeded for migration and invasion assays. I Expressions of Vimentin, E-cadherin and N-cadherin were examined by Immunofluorescence staining. FaDu cells were seeded for Immunofluorescence staining assays at 48 hours post-transfection. J mRNA of Vimentin, E-cadherin and N-cadherin were further examined by RT-PCR assay. FaDu cells were seeded for Immunofluorescence staining assays at 36 hours post-transfection. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01, ns, no significant difference