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Fig. 3 | BMC Cancer

Fig. 3

From: A novel computer-assisted tool for 3D imaging of programmed death-ligand 1 expression in immunofluorescence-stained and optically cleared breast cancer specimens

Fig. 3

Variation in the predicted immune cell score at different depths of the 3D space. (A) Variation of tumor area calculated by the computer-assisted algorithm at different depths of the 3D space. (B) Variation of immune cells calculated by the computer-assisted algorithm at different depths of the 3D space. (C) Variation in the PD-L1 immune cell score calculated by the computer-assisted algorithm at different depths of the 3D space. (D) Maximum observed difference in the immune cell score for different layers (case #16); scale bar = 1000 μm (whole specimen image), scale bar = 200 μm (region of interest image)

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