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Fig. 2 | BMC Cancer

Fig. 2

From: A novel computer-assisted tool for 3D imaging of programmed death-ligand 1 expression in immunofluorescence-stained and optically cleared breast cancer specimens

Fig. 2

Computer-assisted algorithm for the detection of tumor-infiltrating immune cells in immunofluorescence images. (A) The ground truth annotation mask (white) and the tumor cell segmentation model mask (cyan) were visually compared for the detection of tumor areas. (B) The ground truth annotation mask (CD45, green fluorescence) and the immune cell segmentation model mask (yellow mask) were visually compared for the detection of tumor-infiltrating immune cells. (C) Workflow of the computer-assisted algorithm for the detection of PD-L1 expression. Cyan: tumor segmentation model mask; yellow: immune cell segmentation model mask; white: merged tumor area mask; magenta: CD45-positive tumor-infiltrating immune cells mask. Nuclei and cell membranes were counterstained with SYTO-16 (blue color) and DiD (red color), respectively. Scale bar = 2000 μm (whole view), scale bar = 200 μm (region of interest)

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BMC Cancer

ISSN: 1471-2407

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