Fig. 2
From: Notch3 restricts metastasis of breast cancers through regulation of the JAK/STAT5A signaling pathway
STAT5A inhibits the mobility of breast cancer cells by suppressing EMT. a Overexpression of STAT5A caused BT549 cells to lose their long spindle shape. b Immunofluorescence showing that expression of Vimentin was inhibited by STAT5A in BT549 cells. c ImageJ was used to quantitatively analysis the immunofluorescent staining of Vimentin. d Influorescence staining of F-actin in BT549 with STAT5A overexpression and MCF-7 with siSTAT5A. e The colony formation was suppressed by STAT5A overexpression. f Western blot showing in BT549 cells, increased STAT5A expression suppressed the level of the mesenchymal marker, Vimentin. g Wound healing of BT549 cells was suppressed by overexpressing STAT5A. h Invasion of BT549 was suppressed by overexpressing STAT5A. i In MCF-7 cells, suppression of STAT5A levels decreased the expression of the epithelial marker, E-cadherin. j Knockdown of STAT5A promoted wound healing of MCF-7 cells. k Knockdown STAT5A expression promoted invasiveness of MCF-7 cells