Fig. 5
From: Endosulfine alpha maintains spindle pole integrity by recruiting Aurora A during mitosis
ENSA regulates the recruitment of Aurora A to the spindle pole during mitosis. A HeLa cells were transfected with the control or ENSA siRNA for 48 h. Cells were stained with the indicated antibodies. Scale bar, 5 μm. B The percentage of cells with p-Aurora A at the cytosol or spindle pole. HeLa cells were transfected with the control or ENSA siRNA for 48 h. (C and D) PLA of p-ENSA/Aurora A complexes from the control or ENSA-depleted cells. Representative images of the PLA assay (C). Number of foci in mitotic cells (D). Data are presented as the mean ± SEM of values from at least three independent experiments (n = 50 miotic cells for each quantification and group). ***p < 0.0001