Fig. 4

Delay of mitotic entry induced by MKI-2 is restored by PP2A depletion; however, abnormal spindle pole integrity is not affected. A The duration of mitotic entry. Fucci-HeLa cells were transfected with the control, PP2A-B55α siRNA in DMSO (Ctrl), or 20 nM MKI-2 and time-lapse imaged from 48 h after transfection and 24 h after MKI-2 treatment. B-G HeLa cells were treated with DMSO, 20 nM MKI-2, or siRNA of PP2A (5 nM). Quantification of mitotic cells (B). HeLa cells were stained with the indicated antibodies (C and F). Quantification of spindle pole abnormalities during mitosis (D). The percentage of centrosome defects (E). Data are presented as the mean ± SEM of values from at least three independent experiments (n = 50 miotic cells for each quantification and group). ***p < 0.0001. Quantification of p-ENSA intensities (G). The means of values from three separate experiments are shown. Error bars indicate standard deviation (SD). The data represent typical results and are presented as the mean ± SD of values from three independent experiments; ***p < 0.0001; (n = 100 miotic cells for each quantification and group)