Fig. 1

Phosphorylated ENSA localizes at spindle poles during early mitosis. A HeLa or MCF cells stained with the indicated antibodies. Spindles and chromosomes were visualized using α-tubulin (red) and Hoechst (blue), respectively. Scale bar, 5 μm. B and C HeLa cells were transfected with the control or ENSA siRNA (20 nM) for 48 h. Cell lysates were analyzed using immunoblotting with the ENSA antibody (B) or stained with the indicated antibodies. C Scale bar, 5 μm. D Quantification of p-ENSA intensities. The means from three separate experiments are shown. Error bars indicate standard deviation (SD). The data represent typical results and are presented as the mean ± SD of values from three independent experiments; ***, p < 0.0001; (n = 50 miotic cells for each quantification and group)