Fig. 10

B3GNT8 functional verification. A Expression of B3GNT8 mRNA in CFPAC-1 and MIA PaCa-2 cells after knocking down and overexpressing B3GNT8. B Western blot showed B3GNT8 protein expression in CFPAC-1 and MIA PaCa-2 cells after knocking down and overexpressing B3GNT8. The samples are from the same experiment, and the gels/blots were processed in parallel. C Effects of si-B3GNT8 on clonogenic ability of CFPAC-1 (up) and MIA PaCa-2 (down) cells. D Effects of OE-B3GNT8 on clonogenic ability of CFPAC-1 (up) and MIA PaCa-2 (down) cells. E Wound-healing assay showed the migration of CFPAC-1 (left) and MIA PaCa-2 (right) cells were promoted by B3GNT8 knockdown. F Wound-healing assay showed the migration of CFPAC-1 (left) and MIA PaCa-2 (right) cells were inhibited by B3GNT8 overexpression. G Transwell assay showed the invasion of CFPAC-1 (up) and MIA PaCa-2 (down) cells were promoted by B3GNT8 knockdown. H Transwell assay showed the invasion of CFPAC-1 (up) and MIA PaCa-2 (down) cells were inhibited by B3GNT8 overexpression