Fig. 5

Phenotypic analysis of EAC tumor cells in EAC-challenged mice treated with ZnONP nanocompostes. EAC-challenged mice IP inoculated with PBS, DOX (0.4 mg), ZnONPs (0.5 mg), ZnONPs/DOX (0.7 mg), ZnONPs/FA (0.5 mg) or ZnONPs /DOX/FA (0.8 mg). Ascitic EAC cells were harvested from peritoneal cavity and washed twice with PBS. Cells were stained with Propidium Iodide (PI) and annexin V then analyzed by flow cytometry for the marker indicated on the representative histograms