Fig. 4

Potentials of ZnONPs conjugates on the total number of splenocytes in EAC-bearing mice. EAC-bearing mice IP inoculated with EAC-challenged mice IP inoculated with PBS, DOX (0.4 mg), ZnONPs (0.5 mg), ZnONPs/DOX (0.7 mg), ZnONPs/FA (0.5 mg) or ZnONPs /DOX/FA (0.8 mg). Mice were sacrificed on day 11 post tumor implantation and splenocytes were harvested to determine their viability and their total number using trypan blue viability test. Data were represented as mean ± SD (n = 10). Difference between groups was considered statistically significant at P < 0.05. Note: ^a,b,c Statistically significant difference as compared to the corresponding means of the naive group (a), the EAC group (b), the DOX group (c) within each column