Fig. 4
From: Gene delivery to breast cancer by incorporated EpCAM targeted DARPins into AAV2
AAV2MEC1-mediated HSV/TK gene transfer reduces the growth of 4T1 tumor xenografts. A: Schematic showing experimental design in vivo. Female BALB/c mice were subcutaneously injected with 2 × 10^5 4T1 cells in the buttocks. On the third day after tumor inoculation, the mice were divided into three groups. A single intravenous injection of 5 × 10^11 viruses was administered, followed by intraperitoneal administration of GCV (100 mg/kg) 2 days later. This was repeated once every other day for a total of 5 times. The groups included AAV2 (n = 6), AAV2M (n = 6) and AAV2MEC1 (n = 6). B: Measured tumor volumes. The data show that the AAV2MEC1 tumors grew more slowly than the AAV2 and AAV2M tumors. The red arrow indicates the time point of virus injection, and the blue arrow indicates the time point of GCV injection. Tumor volume (mm3) is represented as mean ± SD. C: Percentage survival of AAV2 treated mice (black), AAV2M (blue), AAV2MEC1 (red). D: Representative images of tumors after each mouse was euthanized。E: Accumulated tumor weights are shown for mice receiving AAV2, AAV2M and AAV2MEC1-treated. Data are presented as mean ± SD. *P < 0.05; **P < 0.01; ns statistically not significant