Fig. 5
From: GRB7 plays a promoting role in the progression of gastric cancer
GRB7 positively regulated the expression of MYD88 in gastric cancer. (A) The expression of key proteins of TLR family signaling pathway was detected by western blot. GAPDH was used as the loading control. (B) Grayscale analysis of the protein bands. (C) The expression level of MYD88 was analyzed using the RNA-seq data downloaded from TCGA. (D) Correlation analyses between the expression of GRB7 and MYD88. (E) The binding of GRB7 by MYD88 was verified in AGS sells via a coimmunoprecipitation (Co-IP) assay. Results were presented as mean ± SD. Error bars are the SD for three technical replicates. * P < 0.05, ** P < 0.01, ***P < 0.001