Fig. 9

The m6A modification was enriched in SUCLG2-AS1 and improved its transcripts stability. A The m6A methylation level of SUCLG2-AS1 in AML cells and control cells were determined by MeRIP-qPCR. B The knockdown effect of sh-WTAP was verified by Western blot (WB) analysis in THP-1 cells. C m6A methylation level in THP-1 cells after WTAP was knocked down. D Changes in m6A modified SUCLG2-AS1 levels upon WTAP was knockdown in THP-1 cells. E Transcript levels of WTAP and SUCLG2-AS1 in negative control and sh-WTAP THP-1 cells. F Reduction of SUCLG2-AS1 RNA stability in WTAP knockdown THP-1 cells as compared to control. Cells were treated with actinomycin D and RNA was isolated at 0, 2, and 4 h. Data represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. The experiments were independently repeated at least three times