Fig. 5

The binding between RGD-p21Ras-scFv and p21Ras protein could reduce p21Ras-GTP (active Ras) and inhibit phosphorylation of the downstream pathway MEK-ERK/PI3K-AKT in colorectal cancer. (A) RGD-p21Ras-scFv reduces p21Ras-GTP by western-blot analysis. The colorectal cancer cell lines were cocultured with RGD-p21Ras-scFv, KRAS(G12C) inhibitor, PBS, RGD or DMSO for 48 h. The proteins p21Ras-GTP and total p21Ras were enriched in the cell lysates by an RAS pull-down kit. (B-F) The RGD-p21Ras-scFv inhibits the phosphorylation of the MEK-ERK/PI3K-AKT signaling pathway. Colorectal cancer cell lines were cocultured with RGD-p21Ras-scFv, KRAS(G12C) inhibitor, PBS, RGD or DMSO for 48 h. Cells were lysed, and proteins were extracted for western blot analysis Ras changes in downstream signaling pathways. β-actin was adopted as the control