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Fig. 4 | BMC Cancer

Fig. 4

From: RGD-p21Ras-scFv expressed prokaryotically on a pilot scale inhibits ras-driven colorectal cancer growth by blocking p21Ras-GTP

Fig. 4

RGD-p21Ras-scFv enters tumor cells to bind directly to p21Ras. (A) Immunofluorescence assay showed that RGD-p21Ras-scFv(green) entered tumor cells and co-localized with p21Ras(red) in colorectal cancer cell lines HT29, SW837, LS180, SW480, HCT116. Nuclei were stained with DAPI (blue). (B, C) Pull-down assay revealed that RGD-p21Ras-scFv could directly bind with wild-type K-p21Ras and mutant K-p21Ras. Plasmids of RGD-p21Ras-scFv with Flag (scFv-Flag), blank with Flag (Flag), wild-type KRAS with MYC (K-p21RasW-Myc), and mutant KRAS with MYC (K-p21RasM-Myc) were transfected into 293T cells. No-IP: whole-cell lysate; Flag-IP: eluate by anti-Flag beads, Myc-IP: eluate by anti-Myc beads. No-IP, Flag-IP, and Myc-IP by WB detection

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BMC Cancer

ISSN: 1471-2407

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