Fig. 5
A Flow cytometry analysis for cancer cell markers CD44 and CD24 in CAL33 spheroids without macrophages in single cell suspension on day 7, B Flow cytometry analysis for cancer cell markers CD44 and CD24 in CAL33 spheroids with macrophages in single cell suspension on day 7, C Flow cytometry analysis of CD44 negative cells for M1 macrophage markers (CD14, CD86) and M2 macrophage markers (CD14, CD206 and CD163) in CAL33 spheroids with macrophages in single cell suspension on day 7, D Graphical representation of Flow cytometry data for CD44 and CD24 in CAL33 spheroids with and without macrophages, E Graphical representation of Flow cytometry data for macrophages cell surface markers for M1 and M2 TAMs in CAL33 spheroids with macrophages, F A multicellular spheroid of CAl33 with macrophages representing different zones of TME, G Flow cytometry analysis for M0 macrophage cell surface marker CD14 with M1 macrophage marker (CD14, CD86) and M2 macrophage markers (CD14, CD206 and CD163) on day 7, H Confocal microscopy images of M0 macrophage on day 7 immuno-labelled with M1 macrophage marker CD163 (Green), M2 macrophage marker CD207 (yellow), Macrophage marker CD14 (Red), and nuclei stained with DAPI (blue), I Confocal microscopy image of day 7 CAL33 spheroid without macrophages stained with CFSE-green, J Confocal microscopy image of day 7 CAL33 spheroids with macrophages stained with CFSE-green (cancer cells) and Nile red (macrophage)