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Fig. 3 | BMC Cancer

Fig. 3

From: Macrophage infiltration in 3D cancer spheroids to recapitulate the TME and unveil interactions within cancer cells and macrophages to modulate chemotherapeutic drug efficacy

Fig. 3

A Graph representing CalcineAM mean florescence intensity in single cell suspension of CAL33 spheroids with and without macrophages from day 1 to day 11, B CalcineAM flow cytometry analysis layover histogram of spheroids single cell suspension at day 7 with 3 different population of cells representing cellular health status, C Graph representing Mitospy-green mean florescence intensity for Mitochondrial mass analysis in single cell suspension of spheroids on day 3 to day 11, D Mitospy-green flow cytometry analysis layover histogram of spheroids single cell suspension at day 7 with 3 different population of cells representing different mitochondrial mass and metabolic activity, E Graph representing DAPI mean florescence intensity for dead cell analysis in single cell suspension of spheroids with and without macrophages from day 1 to day 11, F, G Flow cytometry analysis for live cell and dead cell in spheroids single cell suspension at day 7. The measurements were performed for all the conditions in triplicates (n = 3, with 4 spheroids single cell suspension in each condition). Statistical significance was analyzed using two-way ANOVA with Sidak's multiple comparisons test and the p-value obtained are indicated (ns p = 0.1619, * p = 0.0223, ** p = 0.0014, *** p = 0.0003, *** p = 0.0005)

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BMC Cancer

ISSN: 1471-2407

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