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Fig. 1 | BMC Cancer

Fig. 1

From: Macrophage infiltration in 3D cancer spheroids to recapitulate the TME and unveil interactions within cancer cells and macrophages to modulate chemotherapeutic drug efficacy

Fig. 1

A Undifferentiated THP-1 monocytes before treatment with PMA, B THP-1 cells at 48 h incubation with 150 ng/ml of PMA to differentiate monocytes to M0 macrophages, C M0 macrophage at 48 h fixed and immunolabelled with M1 macrophage marker CD163 (Green), M2 macrophage marker CD207 (yellow), Macrophage marker CD14 (Red), and nuclei stained with DAPI (blue), D CalcineAM (Green) and DAPI (Blue) stained CAL33 spheroids without macrophages on different days (at 10X magnification), E CalcineAM (Green) and DAPI (Blue) stained CAL33 spheroids with macrophages on different days (at 10X magnification), F Flow cytometry analysis for M0 macrophage cell surface marker CD14 with M1 macrophage markers (CD14, CD86) and M2 macrophage markers (CD14, CD206 and CD163) on day 2, G. Z- scan confocal microscopy (at 10X magnification) of Spheroids for CalcineAM penetration

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BMC Cancer

ISSN: 1471-2407

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