Fig. 2
From: Discovery of a small molecule that inhibits Bcl-3-mediated cyclin D1 expression in melanoma cells
Identification of the Bcl-3 antagonist that inhibits cyclin D1 expression. A The binding of 4547 3A (BCL3ANT) and 4539 4G to the Bcl-3 ARD causes a significant shift in melting temperature compared to the other compounds. Fluorescence based screening of ligands toward GST-Bcl-3-ARD, where ARD stands for ankyrin repeat domain. Analysis of the melting point for the ligands where the binding of 4547 3A (BCL3ANT) to the protein sample caused the largest shift in melting temperature of 2–3 degrees. B Chemical structure of BCL3ANT consisting of 2-(((4-(3,4-dichlorophenyl)-1,3-thiazol-2-yl) amino) methylene). C The green line represents the control consisting of Bcl-3 ARD protein without the ligand, and the red line represents Bcl-3 ARD in the presence of ligand (BCL3ANT) causing a shift in melting temperature from 51 °C to 54 °C