Fig. 4

Relationship between GLS1 expression and Edmondson–Steiner grade or MVI among patients with HCC (a, d, g, and j). Immunohistochemistry staining of GLS1 in HCC defined as negative (–), weakly positive (+), moderately positive (++), and strongly positive (+++) (b, e, h, and k). Hematoxylin and eosin staining results of Edmondson–Steiner grade I, II, III, and III (c, f, i, and l). Magnification of the area indicated by the black square in (b, e, h, and k). MVI in (i and l) is shown with the black arrow. White bars = 250 μm; black bars = 100 μm