Fig. 4

circZNF800 promotes cell proliferation and sphere and colony formation in CRC cells in vitro. A Effects of circZNF800 on cell proliferation. On circZNF800 over-expression (OE) (left panels) or knockdown (KD) (right panels), the cells were treated with EdU and analyzed by flowcytometry. Representative flowcytometry histographs are shown. Bar charts represent triplicated quantitative analysis. *P < 0.05, **P < 0.01, ***P < 0.001 relative to the respective controls. B Spheroid formation on circZNF800 knockdown. Images were taken after 10 days in the spheroid culture conditions; spheroid quantification is shown (bar represents 100 µm). Colony quantification is also shown. C Colony formation of CRC cells on circZNF800 knockdown. Images show the resulting colonies on staining with a crystal violet solution