Fig. 3

CircZNF800 up-regulates expression of CSC markers in CRC cells. Effects of circZNF800 over-expression (OE) (left panels) and knockdown (KD) (right panels) on the expression levels of the pluripotency markers determined by qRT-PCR (A), the CSC markers CD44 and CD133 (B) and the intestinal stem cell markers LGR5 and SOX9 determined by flowcytometry (C). Representative flowcytometry histograms are shown. Half-offset plots are used to illustrate the shifts of the markers. Scales of 1–0 units used in all cases were algorithmically determined by FlowJo software. Bar charts represent triplicated quantitative analysis. *p < 0.05 and **p < 0.01 were values relative to the scrambled CrSC or vehicle control group