Fig. 8

Negative control labeling of LIM-2405 cells is presented in (A), control (A’), atropine (A’’), and 4-DAMP (A’’’). HT-29 negative control is displayed in (B), control (B’), atropine (B’’), and 4-DAMP (B’’’). Negative control labeling of LIM-2405 cells is presented in (C), control (C’), atropine (C’’), and 4-DAMP (C’’’). HT-29 negative control is displayed in (D), control (D’), atropine (D’’), and 4-DAMP (D’’’). The scale bar represents 50 μm. Bar graphs displaying the mean fluorescence of M3R (E), ChAT (E’); western blot bands for LIM-2405, and HT-29 ran on the same blot with a well separating each cell line, labeled with M3R and ChAT are shown in (F) and western blot expression intensity is shown in (G-G’). C: control, A: atropine, 4-D: 4-DAMP. Data presented as mean ± SEM. Two-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001