Fig. 6

Annexin V-FITC/propidium iodide (PI) staining of human colon cancer cell lines incubated with atropine (A’, B’) and 4-DAMP (A”, B’’). (A) LIM-2405 controls, (A’) LIM-2405 treated with atropine, (A’’) LIM-2405 treated with 4-DAMP, and the percentage of cells treated with control, atropine, and 4-DAMP for LIM-2405 (A”’), (B) HT-29 controls, (B’) HT-29 treated with atropine, (B’’) HT-29 treated with 4-DAMP and the percentage of cells treated with control, atropine and 4-DAMP for HT-29 (B”’). The amount of choline was measured in LIM-2056 (C’) and HT-29 (C’) cells. Values in A-B”’ are mean ± standard error of the mean (SEM) from at least three independent experiments performed in triplicate wells. Values in C-C’ presented as mean ± SEM from at least two independent experiments in duplicates. Two-way ANOVA followed by Tukey’s multiple comparisons test was used, and the significance value is marked with asterisks, *p < 0.05, ***p < 0.001, ****p < 0.0001