Fig. 1

Experimental settings for cell stiffness assessment. (A) Schematic representation of cell micro-indentations using a 5 μm radius spherical cantilever. (B) Representative force-distance curve obtained from AFM indentations for cells. The extend curve that is used for fitting of the Hertz fit model is shown in red. (C) Microscopic pictures of AFM measured cancer cell lines and their corresponding non-malignant counterparts. The cantilever used for measurements is also shown (white arrow). Scale bar (white) represents 100 μm. Abbreviations: AFM – atomic force microscopy