Table 1 Sequences and primers
Name | Sequence (5’-3’) |
|---|---|
Sequences of qPCR primers | |
ACTB real-time Forward | GATCATTGCTCCTCCTGAGC |
ACTB real-time Reverse | ACTCCTGCTTGCTGATCCAC |
WT1 real-time Forward | CCACACCAGGACTCATACAGG |
WT1 real-time Reverse | GATGCATGTTGTGATGGCGG |
Sequences of cloning primers | |
WT1 clone no-tag Forward | CCGGAATTCCTGCAGGACCCGGCTTCC |
WT1 clone no-tag Reverse | CCCGATATCTCAAAGCGCCAGCTGGAGT |
WT1 3’-UTR WT Forward | CTAGCTAGCAAAAGAAAATAGGGGATG |
WT1 3’-UTR WT Reverse | CCGCTCGAGTTGAGAGCAAAGTGAAAA |
WT1 3’-UTR MT Forward | GGGAATTTATTATTTACCGTTCGAACTTTTTACTGTGTAAATATATGTC |
WT1 3’-UTR MT Reverse | GACATATATTTACACAGTAAAAAGTTCGAACGGTAAATAATAAATTCCC |
Sequences of siRNAs | |
NC | UUCUCCGAACGUGUCACGUTT |
siWT1-1 | CCAAAUGACAUCCCAGCUUTT |
siWT1-2 | GGAGACAUACAGGUGUGAATT |
Sequences of miRNAs | |
miR-NC sence | UUCUCCGAACGUGUCACGUTT |
miR-NC anti-sence | ACGUGACACGUUCGGAGAATT |
miR-498-5p sence | UUUCAAGCCAGGGGGCGUUUUUC |
miR-498-5p anti-sence | AAAACGCCCCCUGGCUUGAAAUU |
anti-miR-NC | CAGUACUUUUGUGUAGUACAA |
anti- miR-498-5p | GAAAAACGCCCCCUGGCUUGAAA |