Fig. 7

miR-498-5p is decreased and acts as a tumour suppressor by inhibiting cell proliferation, survival and invasion in NSCLC. (A) qPCR detection of miR-498-5p expression in 60 pairs of NSCLC tissues. Bar value (ΔCtN-ΔCtT) ≥ 1 or ≤ − 1 was considered to be significantly upregulation or downregulation, respectively. Green bars indicated miR-498-5p downregulation (left panel). Comparison of miR-498-5p expression between the same normal (green) and tumour (red) tissues (n = 60) was shown (right panel). **P < 0.01. (B) qPCR quantification of miR-498-5p expression in human non-cancerous Beas-2B, MRC-5 cell lines and seven NSCLC cell lines. (C) qPCR quantification of miR-498-5p expression in two pairs of differential metastatic NSCLC cell lines (95 C vs. 95D, NL9980 vs. L9981). (D, E) Growth curves of the indicated cells assessed by CCK-8 assays. (F, G) Colony formation results. Representative images (left) and quantification graph (right) were provided. (H, I) Transwell invasion results. Representative images (left) and quantification graph (right) were provided. **P < 0.01, ***P < 0.001. Scale bar, 200 μm