Fig. 6

WT1 is a direct target of miR-498-5p and their expression inversely correlate with each other. (A) Schematic depicting of WT1 3’ UTR containing the conserved binding site of miR-498-5p (marked by a yellow background) across multiple species. (B) Target candidates of miR-498-5p were predicted using four bioinformatic tools: miRDB, miRwalk, Targetscan and miRmap. (C) Schematic diagram of constructing the wild type and mutant type WT 3’ UTR luciferase reporters. (D) Dual luciferase assay results in A549 and H1299 cells showing a direct targeting of WT1 by miR-498-5p. (E) qPCR detection of miR-498-5p and WT1 expression after transfection with miR-498-5p mimics. (F) Western blot detection of WT1 expression as transfected in (E). (G) qPCR detection of miR-498-5p and WT1 expression after transfection with miR-498-5p inhibitor (anti-miR-498-5p). (H) Western blot detection of WT1 expression as transfected in (G). (I) Correlation analysis of WT1 and miR-498-5p expression in 60 pairs of NSCLC tissues assessed by Spearman’s rank correlation analysis. *P < 0.05, **P < 0.01, ***P < 0.001. NS, no significant