Fig. 3
WT1 is overexpressed in NSCLC tissues and cell lines. (A) qPCR detection of WT1 mRNA expression in 60 pairs of NSCLC tissues. ΔCtN: threshold cycle (Ct) value of β-actin was subtracted from the Ct value of WT1 of paired normal tissue. ΔCtT: Ct value of β-actin was subtracted from that of WT1 of NSCLC tissue. Bar value (ΔCtN-ΔCtT) ≥ 1 or ≤ − 1 was considered to be significantly upregulation or downregulation, respectively. Red bars indicated WT1 upregulation (left panel). Comparison of WT1 mRNA expression between the same normal (green) and tumour (red) tissues (n = 60) was completed by calculating ΔCtβ−actin-ΔCtWT1 (right panel). **P < 0.01. (B) Western blot (upper) and quantification (bottom) of WT1 expression in 24 pairs of NSCLC tissues. Loading control: β-actin. Ratio of WT1/β-actin was calculated and + indicated upregulation (bottom, left). Group comparison result was also provided (bottom, right). **P < 0.01. (C) Western blot of WT1 expression in the non-cancerous Beas-2B, MRC-5 cell lines and seven NSCLC cell lines. (D) Western blot of WT1 expression in two pairs of differential metastatic NSCLC cell lines (95 C vs. 95D, NL9980 vs. L9981)