Fig. 5

ATM silencing induces ROC accumulation and HIF-1α expression in PC cell lines. Intracellular ROS levels were measured after transfection with si-ATM and si-ATM negative control in PC cell lines, MIA-PaCa2 (A; left), and SUIT-2 (A; right) cells. Cells treated with 2 µg/mL lipopolysaccharide for 24 h were used as positive control. HIF-1α and p-HIF-1α expression after ATM-knockdown in MIA-PaCa2 (B; left) and SUIT-2 (B; right) was measured by western blotting and quantified using ImageJ software. For the original bands, please see additional files Supplementary Fig. S5 and Fig. S6. All data are presented as mean ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. PC, pancreatic cancer; ATM, ataxia telangiectasia mutated; si-con/si-1/si-2, ATM si-RNA negative control/1/2; HIF-1α, hypoxia inducible factor-1α; p-HIF-1α, phosphorylated hypoxia inducible factor-1α