Fig. 4

HIF-1α, NTN1, BCL-2, and BAD expression in ATM-knockdown PC cell lines. RT-qPCR analysis of MET mRNA expression after transfection with si-ATM and si-ATM negative control in MIA-PaCa2 (A left), and SUIT-2 (A right) cells. RT-qPCR analysis of NTN1 mRNA expression after transfection with si-ATM and si-ATM negative control in PC cell lines, MIA-PaCa2 (B left), and SUIT-2 (B right) cells. NTN1, MET, BCL-2, and BAD expression after ATM-knockdown was measured by western blotting and quantified using ImageJ software in MIA-PaCa2 (C), and SUIT-2 (D) cells. For the original bands, please see additional files Supplementary Fig. S3 and Fig. S4. All data are presented as mean ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. PC, pancreatic cancer; ATM, ataxia telangiectasia mutated; si-con/si-1/si-2, ATM si-RNA negative control/1/2; MET, mesenchymal-epithelial transition; NTN1, netrin-1; BCL-2, B cell lymphoma-2; BAD, Bcl-2 agonist of cell death