Fig. 2

Expression of ATM in PC cell lines. RT-qPCR analysis of ATM mRNA expression after transfection with si-ATM and si-ATM negative control, respectively, in PC MIA-PaCa2 (A; left) and SUIT-2 (A; right) cell lines. After transfection with si-ATM and si-ATM negative control, ATM and p-ATM expression in MIA-PaCa2 (B; left) and SUIT-2 (B; right) cells was measured by western blotting and quantified using ImageJ software. For the original bands, please see additional file Supplementary Fig. S2. Cell-proliferation assay using MIA-PaCa2 (C; left) and SUIT-2 cells (c; right). Migration activities were assessed using a wound-healing assay (D). All data are presented as mean ± SD from three independent experiments. ****p < 0.0001. PC, pancreatic cancer; ATM, ataxia telangiectasia mutated; p-ATM, phosphorylated ATM. si-con/si-ATM1/si-ATM2, si-ATM RNA negative control/1/2