Skip to main content
Fig. 4 | BMC Cancer

Fig. 4

From: Targeting sphingolipid metabolism with the sphingosine kinase inhibitor SKI-II overcomes hypoxia-induced chemotherapy resistance in glioblastoma cells: effects on cell death, self-renewal, and invasion

Fig. 4

SKI-II alone and in combination with temozolomide (TMZ) increases cytoplasmic vacuolization in glioblastoma cells. A Light microscopy of NCH82 cells treated for 72 h at 3% O2 with the vehicle control (DMSO), 48 µM TMZ, 2.66 µM SKI-II, and the combination (TMZ + SKI-II). B-E NCH82 cells were treated accordingly for 24- to 72 h under normoxia (21% O2) and hypoxia (3% O2) and further analyzed by flow cytometry. The light scattering parameters SSC (side scatter) and FSC (forward scatter) were quantified with FlowJo. Data represent mean (+ SD) of n = 3. Scale bar: 100 µm. Two-way ANOVA followed by Šídák's multiple comparisons test was performed for statistical analysis

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us