Fig. 5

RTK/KRAS and VEGF-A/VEGFR-2 inhibition CD44( +) GA cells blocks metastasis. A Representative H&E photos of mouse lungs 3.5 weeks after tail vein injection of CD44( +), CD44(-), and unsorted Tcon3077 cells. Graph showing number of metastases > 100m per field for each group. B Graph showing bioluminescence after IVIS imaging of mouse lungs 3.5 weeks after tail vein injection of Tcon3077 cells stably transduced with KRAS shRNA, VEGF-A shRNA, or both. Scramble shRNA was used as a control. C H&E and IF photos (YFP, yellow; DAPI, blue) of mouse lungs. Graphs show number of metastatic foci > 100m per field and percent of YFP-positive cells. D H&E, VEGF-A IHC, and VEGF-A (green)/CD31 (white)/CD44 (red) IF. Graphs showing the percent of CD44, VEGF-A, and CD31-expressing cells. Bars represent standard deviation. *p < 0.05 compared to control. **p < 0.05 compared to all other groups