Fig. 4

Inhibition of RTK-RAS and VEGF-A/VEGFR2 blocks tumorigenesis in an orthotropic model of GA. A Western blot analysis for KRAS, VEGF-A and β-actin in CD44( +) Tcon3077 cells stably transduced with KRAS shRNA (sh.KRAS), VEGF-A shRNA (sh.VEGF-A) and/or control scramble shRNA (Original version in Suppl. Figs. S6B-C). B IVIS images 10 weeks after intra-gastric injection of CD44( +) Tcon3077 cells transduced with sh.KRAS and/or sh.VEGF-A. Graph showing bioluminescence for each group. C Representative gross photos and H&E slides of stomach tumors in mice sacrificed at 12 weeks. D IF images of gastric tumors stained for CD44 (red), CD31 (white), and p-MEK1/2 (green). Graphs showing number of positive cells. E Survival curve of 4 groups of mice. Dashed line represents time of sacrifice (12weeks) of two mice for stomach tissue analysis. Bars represent standard deviation. *p < 0.05 compared to control. **p < 0.05 compared to all other groups