Fig. 2

KRAS promotes GA spheroid cell secretion of VEGF-A, FGF-2, and/or EGF. Graphs showing levels of secreted angiogenic factors into basal media devoid of supplements from Tcon3944 monolayer cells and spheroids A from Tcon3944 spheroids transduced with KRAS shRNA (sh.KRAS) and control scramble shRNA (sh.Scr). B as determined by the Proteome Profiler Mouse Angiogenesis Array kit. Graphs showing levels of secreted angiogenic factors from SNU668 monolayer cells and spheroids C and from SNU668 spheroids transduced with sh.KRAS and sh.Scr D as determined by the Proteome Profiler Human Angiogenesis Array kit. E Graph of tube formation with conditioned media from AGS and MKN-45 spheroids treated with sh.KRAS, sh.Scr, recombinant EGF (rEGF), recombinant VEGF-A (rVEGF-A), or recombinant FGF-2 (rFGF-2). Bars represent standard deviation. *p < 0.05 compared to control